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dual color elispot kit  (R&D Systems)


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    R&D Systems dual color elispot kit
    Dual Color Elispot Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual color elispot kit/product/R&D Systems
    Average 93 stars, based on 9 article reviews
    dual color elispot kit - by Bioz Stars, 2026-06
    93/100 stars

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    FIGURE 2 | Total and antigen-specific IgG-secreting B cells in steroid-sensitive nephrotic syndrome pediatric patients at onset. (A–E) Isolated PBMCs were stimulated for 5 days with CpG plus rhIL-21 and rhIL-4. Following stimulation, (A) total, (B,C) anti-tetanus and (D,E) anti-HBV IgG-secreting B cells were enumerated by <t>ELISPOT</t> in steroid-sensitive nephrotic syndrome pediatric patients at disease onset (SSNS, n=11) and in age-matched controls (CTRL, n=5). Antigen-specific memory B cells were represented as (B,D) absolute count/106 cells and as (C,E) percentage of total IgG-secreting B cells. Each plot represents a different patient. Horizontal lines indicate the means and differences between groups were compared using the unpaired t test.
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    FIGURE 2 | Total and antigen-specific IgG-secreting B cells in steroid-sensitive nephrotic syndrome pediatric patients at onset. (A–E) Isolated PBMCs were stimulated for 5 days with CpG plus rhIL-21 and rhIL-4. Following stimulation, (A) total, (B,C) anti-tetanus and (D,E) anti-HBV IgG-secreting B cells were enumerated by ELISPOT in steroid-sensitive nephrotic syndrome pediatric patients at disease onset (SSNS, n=11) and in age-matched controls (CTRL, n=5). Antigen-specific memory B cells were represented as (B,D) absolute count/106 cells and as (C,E) percentage of total IgG-secreting B cells. Each plot represents a different patient. Horizontal lines indicate the means and differences between groups were compared using the unpaired t test.

    Journal: Frontiers in immunology

    Article Title: Evaluation of Immune and Vaccine Competence in Steroid-Sensitive Nephrotic Syndrome Pediatric Patients.

    doi: 10.3389/fimmu.2021.602826

    Figure Lengend Snippet: FIGURE 2 | Total and antigen-specific IgG-secreting B cells in steroid-sensitive nephrotic syndrome pediatric patients at onset. (A–E) Isolated PBMCs were stimulated for 5 days with CpG plus rhIL-21 and rhIL-4. Following stimulation, (A) total, (B,C) anti-tetanus and (D,E) anti-HBV IgG-secreting B cells were enumerated by ELISPOT in steroid-sensitive nephrotic syndrome pediatric patients at disease onset (SSNS, n=11) and in age-matched controls (CTRL, n=5). Antigen-specific memory B cells were represented as (B,D) absolute count/106 cells and as (C,E) percentage of total IgG-secreting B cells. Each plot represents a different patient. Horizontal lines indicate the means and differences between groups were compared using the unpaired t test.

    Article Snippet: For the simultaneous detection of IgM and IgG we used the Human IgG/IgM Dual-Color B Cell ELISpot Kit (R&D System).

    Techniques: Isolation, Enzyme-linked Immunospot